rabbit anti p2y2 (Alomone Labs)
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Rabbit Anti P2y2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p2y2/Anti-P2Y2+Receptor+Antibody/10__1016_slash_j__ghres__2026__100021-55-3-6
Average 95 stars, based on 90 article reviews
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Blocking Assay:Article Title: Purinergic GPCR-integrin interactions drive pancreatic cancer cell invasion Article Snippet: Gels were run at 150 V for 2 hr and transferred into a nitrocellulose membrane (GE Healthcare) at 100 V for 1 hr. .. Following blocking with 5% milk (Sigma) in 0.1% TBS- T for 1 hr, membranes were incubated with 1:1000 dilution of antibodies against phosphorylated FAK (Tyr397, 3283, Cell Signaling, RRID:AB_2173659), phosphorylated ERK 1/2 (S217/221, 9154, Cell Signaling, RRID:AB_2138017), Article Title: Analysis of purine receptor expression and functionality in alveolar epithelial cells. Article Snippet: The samples were separated by a 4–12% Bis-Tris polyacrylamide gel (NuPage, Life Technologies GmbH) and transferred to nitrocellulose filters (Life Technologies GmbH) by electroblotting. .. After addition of blocking buffer (PBS plus 0.1% Tween containing 5% milk powder) followed by agitation for 1 h, specific P2R antibodies were applied overnight at 4 °C ( Article Title: Alterations of Purinergic Receptors Levels and Their Involvement in the Glial Cell Morphology in a Pre-Clinical Model of Autism Spectrum Disorders Article Snippet: P2Y1 , Sigma-Aldrich, St. Louis, MO, USA , P-6487 , Blocking: 5% milk in TBS-T, 1 h at RT I-ab 1:100 in TBS-T, overnight at 4 °C II-ab 1:4000 in 5% milk in TBS-T, 1 h in RT. .. Incubation:Article Title: Purinergic GPCR-integrin interactions drive pancreatic cancer cell invasion Article Snippet: Gels were run at 150 V for 2 hr and transferred into a nitrocellulose membrane (GE Healthcare) at 100 V for 1 hr. .. Following blocking with 5% milk (Sigma) in 0.1% TBS- T for 1 hr, membranes were incubated with 1:1000 dilution of antibodies against phosphorylated FAK (Tyr397, 3283, Cell Signaling, RRID:AB_2173659), phosphorylated ERK 1/2 (S217/221, 9154, Cell Signaling, RRID:AB_2138017), Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish Article Snippet: .. All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: Article Title: Effect of cellular senescence on the P2Y-receptor mediated calcium response in trabecular meshwork cells Article Snippet: Proteins were separated by 10% SDS–PAGE and transferred to PVDF membrane (BioRad, Hercules, CA). .. Membranes were blocked with 5% non-fat dry milk and incubated overnight with specific antibodies against P2Y1, Article Title: Astrocyte-mediated purinergic signaling is upregulated in a mouse model of Fragile X syndrome. Article Snippet: Funding information Canadian Institutes of Health Research; Fondation Brain Canada; FRAXA Research Foundation Abstract Fragile X syndrome (FXS) is the leading monogenic cause of intellectual disability and autism spectrum disorders.. With increasing investigation into the molecular mechanisms underlying FXS, there is growing evidence that perturbations in glial signaling are widely associated with neurological pathology.. Purinergic signaling, which utilizes nucleoside triphosphates as signaling molecules, provides one of the most ubiquitous signaling systems for glial-neuronal and glial-glial crosstalk. Tissue:Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish Article Snippet: .. All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: Saline:Article Title: Astrocyte-mediated purinergic signaling is upregulated in a mouse model of Fragile X syndrome. Article Snippet: Funding information Canadian Institutes of Health Research; Fondation Brain Canada; FRAXA Research Foundation Abstract Fragile X syndrome (FXS) is the leading monogenic cause of intellectual disability and autism spectrum disorders.. With increasing investigation into the molecular mechanisms underlying FXS, there is growing evidence that perturbations in glial signaling are widely associated with neurological pathology.. Purinergic signaling, which utilizes nucleoside triphosphates as signaling molecules, provides one of the most ubiquitous signaling systems for glial-neuronal and glial-glial crosstalk. Transcranial Magnetic Stimulation:Article Title: Astrocyte-mediated purinergic signaling is upregulated in a mouse model of Fragile X syndrome. Article Snippet: Funding information Canadian Institutes of Health Research; Fondation Brain Canada; FRAXA Research Foundation Abstract Fragile X syndrome (FXS) is the leading monogenic cause of intellectual disability and autism spectrum disorders.. With increasing investigation into the molecular mechanisms underlying FXS, there is growing evidence that perturbations in glial signaling are widely associated with neurological pathology.. Purinergic signaling, which utilizes nucleoside triphosphates as signaling molecules, provides one of the most ubiquitous signaling systems for glial-neuronal and glial-glial crosstalk. Article Title: Alterations of Purinergic Receptors Levels and Their Involvement in the Glial Cell Morphology in a Pre-Clinical Model of Autism Spectrum Disorders Article Snippet: P2Y1 , Sigma-Aldrich, St. Louis, MO, USA , P-6487 , Blocking: 5% milk in TBS-T, 1 h at RT I-ab 1:100 in TBS-T, overnight at 4 °C II-ab 1:4000 in 5% milk in TBS-T, 1 h in RT. .. other:Article Title: Purinergic Signalling Mediates Aberrant Excitability of Developing Neuronal Circuits in the Fmr1 Knockout Mouse Model. Article Snippet: During development, the formation of appropriate neuronal connections is heavily dependent on the intricate choreography of neuronal and glial interactions.. Astrocytes are highly prevalent CNS glial cells and key participants of these early neurodevelopmental processes.. It is therefore not surprising that defects to normal astrocyte function have been associated with several neurodevelopmental diseases, including autism spectrum disorders (ASDs). |
