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rabbit anti p2y2  (Alomone Labs)


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    Alomone Labs rabbit anti p2y2
    Rabbit Anti P2y2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p2y2/Anti-P2Y2+Receptor+Antibody/10__1016_slash_j__ghres__2026__100021-55-3-6
    Average 95 stars, based on 90 article reviews
    rabbit anti p2y2 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Purinergic GPCR-integrin interactions drive pancreatic cancer cell invasion
    Article Snippet: Gels were run at 150 V for 2 hr and transferred into a nitrocellulose membrane (GE Healthcare) at 100 V for 1 hr. .. Following blocking with 5% milk (Sigma) in 0.1% TBS- T for 1 hr, membranes were incubated with 1:1000 dilution of antibodies against phosphorylated FAK (Tyr397, 3283, Cell Signaling, RRID:AB_2173659), phosphorylated ERK 1/2 (S217/221, 9154, Cell Signaling, RRID:AB_2138017), P2Y2 (APR- 010, Alomone Labs, RRID:AB_2040078), HSC 70 (SC7298, Santa Cruz, RRID:AB_627761), or α-tubulin (T5168, Sigma- Aldrich, RRID:AB_477579) with 5% BSA in 0.1% TBS- T overnight at 4 °C. .. Gels were run at 150 V for 2 hr and transferred into a nitrocellulose membrane (GE Healthcare) at 100 V for 1 hr.

    Article Title: Analysis of purine receptor expression and functionality in alveolar epithelial cells.
    Article Snippet: The samples were separated by a 4–12% Bis-Tris polyacrylamide gel (NuPage, Life Technologies GmbH) and transferred to nitrocellulose filters (Life Technologies GmbH) by electroblotting. .. After addition of blocking buffer (PBS plus 0.1% Tween containing 5% milk powder) followed by agitation for 1 h, specific P2R antibodies were applied overnight at 4 °C (P2Y2, P2Y4, P2Y6, P2Y12, P2Y13, P2X3, P2X4, P2X5, and P2X7 from Alomone Labs, Jerusalem, Israel; P2Y1 from Genway, San Diego, CA, USA; and P2Y14 from Abcam, Cambridge, UK). .. Bound primary antibodies were visualized using horseradish peroxidase-conjugated secondary antibodies (1 h at room temperature with agitation) and enhanced chemiluminescent substrate (ECL) reagent (ECL Plus Western Blotting Detection Reagents, GE Healthcare, Freiburg, Germany) according to the manufacturer’s protocol.

    Article Title: Alterations of Purinergic Receptors Levels and Their Involvement in the Glial Cell Morphology in a Pre-Clinical Model of Autism Spectrum Disorders
    Article Snippet: P2Y1 , Sigma-Aldrich, St. Louis, MO, USA , P-6487 , Blocking: 5% milk in TBS-T, 1 h at RT I-ab 1:100 in TBS-T, overnight at 4 °C II-ab 1:4000 in 5% milk in TBS-T, 1 h in RT. .. P2Y2 , Alomone Labs, Jerusalem, Israel , APR-010 , Blocking: 5% milk in TBS-T, 1 h at RT I-ab 1:100 in 2% BSA, overnight at 4 °C II-ab 1:4000 in 5% milk in TBS-T, 1 h at RT. .. P2Y12 , Thermo Fischer, Carlsbad, CA, USA , 702516 , Blocking: 5% BSA in TBS-T, 1 h at RT I-ab 1:1000 in 5% milk in TBS-T, overnight at 4 °C II-ab 1:1000 in 5% milk in TBS-T, 1 h at RT.

    Incubation:

    Article Title: Purinergic GPCR-integrin interactions drive pancreatic cancer cell invasion
    Article Snippet: Gels were run at 150 V for 2 hr and transferred into a nitrocellulose membrane (GE Healthcare) at 100 V for 1 hr. .. Following blocking with 5% milk (Sigma) in 0.1% TBS- T for 1 hr, membranes were incubated with 1:1000 dilution of antibodies against phosphorylated FAK (Tyr397, 3283, Cell Signaling, RRID:AB_2173659), phosphorylated ERK 1/2 (S217/221, 9154, Cell Signaling, RRID:AB_2138017), P2Y2 (APR- 010, Alomone Labs, RRID:AB_2040078), HSC 70 (SC7298, Santa Cruz, RRID:AB_627761), or α-tubulin (T5168, Sigma- Aldrich, RRID:AB_477579) with 5% BSA in 0.1% TBS- T overnight at 4 °C. .. Gels were run at 150 V for 2 hr and transferred into a nitrocellulose membrane (GE Healthcare) at 100 V for 1 hr.

    Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish
    Article Snippet: .. All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448). .. The sections were then washed three times in 0.01 M PBS for 15 min each before a 2‐h incubation in 0.1% Triton X‐100/0.01 M PBS solution containing one of the following secondary antibodies: Anti‐mouse (Goat, FITC, 1:100, Jackson ImmunoResearch: 115‐095‐166, RRID: AB_2338601), anti‐chicken (Donkey, FITC, 1:200, Jackson ImmunoResearch: 703‐095‐155, RRID: AB_2340356), or anti‐rabbit (donkey, AlexaFluor 568, 1:500, Invitrogen: A10042, RRID: AB_2338601) at room temperature and protected from light.

    Article Title: Effect of cellular senescence on the P2Y-receptor mediated calcium response in trabecular meshwork cells
    Article Snippet: Proteins were separated by 10% SDS–PAGE and transferred to PVDF membrane (BioRad, Hercules, CA). .. Membranes were blocked with 5% non-fat dry milk and incubated overnight with specific antibodies against P2Y1, P2Y2, and P2Y4 receptors (Alomone Laboratories, Jerusalem, Israel). .. Immunoreactive bands were detected by incubation with a secondary antibody conjugated to horseradish peroxidase and chemiluminescence substrate (SuperSignal, West Pico; Pierce, Rockford, IL).

    Article Title: Astrocyte-mediated purinergic signaling is upregulated in a mouse model of Fragile X syndrome.
    Article Snippet: Funding information Canadian Institutes of Health Research; Fondation Brain Canada; FRAXA Research Foundation Abstract Fragile X syndrome (FXS) is the leading monogenic cause of intellectual disability and autism spectrum disorders.. With increasing investigation into the molecular mechanisms underlying FXS, there is growing evidence that perturbations in glial signaling are widely associated with neurological pathology.. Purinergic signaling, which utilizes nucleoside triphosphates as signaling molecules, provides one of the most ubiquitous signaling systems for glial-neuronal and glial-glial crosstalk.

    Tissue:

    Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish
    Article Snippet: .. All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448). .. The sections were then washed three times in 0.01 M PBS for 15 min each before a 2‐h incubation in 0.1% Triton X‐100/0.01 M PBS solution containing one of the following secondary antibodies: Anti‐mouse (Goat, FITC, 1:100, Jackson ImmunoResearch: 115‐095‐166, RRID: AB_2338601), anti‐chicken (Donkey, FITC, 1:200, Jackson ImmunoResearch: 703‐095‐155, RRID: AB_2340356), or anti‐rabbit (donkey, AlexaFluor 568, 1:500, Invitrogen: A10042, RRID: AB_2338601) at room temperature and protected from light.

    Saline:

    Article Title: Astrocyte-mediated purinergic signaling is upregulated in a mouse model of Fragile X syndrome.
    Article Snippet: Funding information Canadian Institutes of Health Research; Fondation Brain Canada; FRAXA Research Foundation Abstract Fragile X syndrome (FXS) is the leading monogenic cause of intellectual disability and autism spectrum disorders.. With increasing investigation into the molecular mechanisms underlying FXS, there is growing evidence that perturbations in glial signaling are widely associated with neurological pathology.. Purinergic signaling, which utilizes nucleoside triphosphates as signaling molecules, provides one of the most ubiquitous signaling systems for glial-neuronal and glial-glial crosstalk.

    Transcranial Magnetic Stimulation:

    Article Title: Astrocyte-mediated purinergic signaling is upregulated in a mouse model of Fragile X syndrome.
    Article Snippet: Funding information Canadian Institutes of Health Research; Fondation Brain Canada; FRAXA Research Foundation Abstract Fragile X syndrome (FXS) is the leading monogenic cause of intellectual disability and autism spectrum disorders.. With increasing investigation into the molecular mechanisms underlying FXS, there is growing evidence that perturbations in glial signaling are widely associated with neurological pathology.. Purinergic signaling, which utilizes nucleoside triphosphates as signaling molecules, provides one of the most ubiquitous signaling systems for glial-neuronal and glial-glial crosstalk.

    Article Title: Alterations of Purinergic Receptors Levels and Their Involvement in the Glial Cell Morphology in a Pre-Clinical Model of Autism Spectrum Disorders
    Article Snippet: P2Y1 , Sigma-Aldrich, St. Louis, MO, USA , P-6487 , Blocking: 5% milk in TBS-T, 1 h at RT I-ab 1:100 in TBS-T, overnight at 4 °C II-ab 1:4000 in 5% milk in TBS-T, 1 h in RT. .. P2Y2 , Alomone Labs, Jerusalem, Israel , APR-010 , Blocking: 5% milk in TBS-T, 1 h at RT I-ab 1:100 in 2% BSA, overnight at 4 °C II-ab 1:4000 in 5% milk in TBS-T, 1 h at RT. .. P2Y12 , Thermo Fischer, Carlsbad, CA, USA , 702516 , Blocking: 5% BSA in TBS-T, 1 h at RT I-ab 1:1000 in 5% milk in TBS-T, overnight at 4 °C II-ab 1:1000 in 5% milk in TBS-T, 1 h at RT.

    other:

    Article Title: Purinergic Signalling Mediates Aberrant Excitability of Developing Neuronal Circuits in the Fmr1 Knockout Mouse Model.
    Article Snippet: During development, the formation of appropriate neuronal connections is heavily dependent on the intricate choreography of neuronal and glial interactions.. Astrocytes are highly prevalent CNS glial cells and key participants of these early neurodevelopmental processes.. It is therefore not surprising that defects to normal astrocyte function have been associated with several neurodevelopmental diseases, including autism spectrum disorders (ASDs).



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    Protein changes and transcriptional expression of <t>P2Y2</t> following injury. (A) For a naïve/non‐injured fish, 2 mm of thoracic spinal cord was collected. (B) For injured fish, 2 mm of spinal cord was collected containing the injury site, as well as 2 mm immediately adjacent on each side (Ro = Rostral, Ca = Caudal). (C) Representative western blot and corresponding total protein showing naïve zebrafish (Zf) spinal cord tissue, de‐glycosylated (Dg) naïve zebrafish spinal cord, mouse brain tissue as a positive control (+), and naïve zebrafish spinal cord tissue pre‐absorbed with a blocking peptide as a negative control (−). (D, F, H) Representative western blot and corresponding total protein at 7 dpi, 14 dpi, and 21 dpi, respectively. (E) Quantitative analysis showing significant upregulation of the 50 kDa isoform within the rostral ( p < 0.0001), injury ( p = 0.0119), and caudal site ( p = 0.0002) at 7 dpi when normalized to naïve tissue. (G) Quantitative analysis of P2Y2 showing basal levels of expression at 14 dpi when normalized to naïve tissue. (I) Quantitative analysis showing significant downregulation of the 25 kDa isoform within the rostral ( p = 0.0073), injury ( p = 0.0015), and caudal site ( p = 0.0002) at 21 dpi when normalized to naïve tissue. (J) Heat map showing RNA‐seq analysis of P2Y2 gene transcripts compared to highly up‐ and down‐regulated genes in rostral and caudal tissue at 7 and 14 dpi. (K, L) Volcano plot of the zebrafish transcriptome at 7 dpi with P2Y2 transcripts and highly up‐ and down‐regulated genes labeled in rostral and caudal tissue, respectively. Total protein used as loading control. One‐way ANOVA with Dunnett's post hoc test was used for western blot analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.005. ***significant differences: p ≤ 0.001. For RNA‐seq analysis, calculated adjusted p ‐values < 0.05 and a log2(fold change) < −1 or > 1 were considered differentially expressed. **** Significant differences: p ≤ 0.0001.
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    Protein changes and transcriptional expression of <t>P2Y2</t> following injury. (A) For a naïve/non‐injured fish, 2 mm of thoracic spinal cord was collected. (B) For injured fish, 2 mm of spinal cord was collected containing the injury site, as well as 2 mm immediately adjacent on each side (Ro = Rostral, Ca = Caudal). (C) Representative western blot and corresponding total protein showing naïve zebrafish (Zf) spinal cord tissue, de‐glycosylated (Dg) naïve zebrafish spinal cord, mouse brain tissue as a positive control (+), and naïve zebrafish spinal cord tissue pre‐absorbed with a blocking peptide as a negative control (−). (D, F, H) Representative western blot and corresponding total protein at 7 dpi, 14 dpi, and 21 dpi, respectively. (E) Quantitative analysis showing significant upregulation of the 50 kDa isoform within the rostral ( p < 0.0001), injury ( p = 0.0119), and caudal site ( p = 0.0002) at 7 dpi when normalized to naïve tissue. (G) Quantitative analysis of P2Y2 showing basal levels of expression at 14 dpi when normalized to naïve tissue. (I) Quantitative analysis showing significant downregulation of the 25 kDa isoform within the rostral ( p = 0.0073), injury ( p = 0.0015), and caudal site ( p = 0.0002) at 21 dpi when normalized to naïve tissue. (J) Heat map showing RNA‐seq analysis of P2Y2 gene transcripts compared to highly up‐ and down‐regulated genes in rostral and caudal tissue at 7 and 14 dpi. (K, L) Volcano plot of the zebrafish transcriptome at 7 dpi with P2Y2 transcripts and highly up‐ and down‐regulated genes labeled in rostral and caudal tissue, respectively. Total protein used as loading control. One‐way ANOVA with Dunnett's post hoc test was used for western blot analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.005. ***significant differences: p ≤ 0.001. For RNA‐seq analysis, calculated adjusted p ‐values < 0.05 and a log2(fold change) < −1 or > 1 were considered differentially expressed. **** Significant differences: p ≤ 0.0001.
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    Image Search Results


    Protein changes and transcriptional expression of P2Y2 following injury. (A) For a naïve/non‐injured fish, 2 mm of thoracic spinal cord was collected. (B) For injured fish, 2 mm of spinal cord was collected containing the injury site, as well as 2 mm immediately adjacent on each side (Ro = Rostral, Ca = Caudal). (C) Representative western blot and corresponding total protein showing naïve zebrafish (Zf) spinal cord tissue, de‐glycosylated (Dg) naïve zebrafish spinal cord, mouse brain tissue as a positive control (+), and naïve zebrafish spinal cord tissue pre‐absorbed with a blocking peptide as a negative control (−). (D, F, H) Representative western blot and corresponding total protein at 7 dpi, 14 dpi, and 21 dpi, respectively. (E) Quantitative analysis showing significant upregulation of the 50 kDa isoform within the rostral ( p < 0.0001), injury ( p = 0.0119), and caudal site ( p = 0.0002) at 7 dpi when normalized to naïve tissue. (G) Quantitative analysis of P2Y2 showing basal levels of expression at 14 dpi when normalized to naïve tissue. (I) Quantitative analysis showing significant downregulation of the 25 kDa isoform within the rostral ( p = 0.0073), injury ( p = 0.0015), and caudal site ( p = 0.0002) at 21 dpi when normalized to naïve tissue. (J) Heat map showing RNA‐seq analysis of P2Y2 gene transcripts compared to highly up‐ and down‐regulated genes in rostral and caudal tissue at 7 and 14 dpi. (K, L) Volcano plot of the zebrafish transcriptome at 7 dpi with P2Y2 transcripts and highly up‐ and down‐regulated genes labeled in rostral and caudal tissue, respectively. Total protein used as loading control. One‐way ANOVA with Dunnett's post hoc test was used for western blot analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.005. ***significant differences: p ≤ 0.001. For RNA‐seq analysis, calculated adjusted p ‐values < 0.05 and a log2(fold change) < −1 or > 1 were considered differentially expressed. **** Significant differences: p ≤ 0.0001.

    Journal: The FASEB Journal

    Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

    doi: 10.1096/fj.202504890RR

    Figure Lengend Snippet: Protein changes and transcriptional expression of P2Y2 following injury. (A) For a naïve/non‐injured fish, 2 mm of thoracic spinal cord was collected. (B) For injured fish, 2 mm of spinal cord was collected containing the injury site, as well as 2 mm immediately adjacent on each side (Ro = Rostral, Ca = Caudal). (C) Representative western blot and corresponding total protein showing naïve zebrafish (Zf) spinal cord tissue, de‐glycosylated (Dg) naïve zebrafish spinal cord, mouse brain tissue as a positive control (+), and naïve zebrafish spinal cord tissue pre‐absorbed with a blocking peptide as a negative control (−). (D, F, H) Representative western blot and corresponding total protein at 7 dpi, 14 dpi, and 21 dpi, respectively. (E) Quantitative analysis showing significant upregulation of the 50 kDa isoform within the rostral ( p < 0.0001), injury ( p = 0.0119), and caudal site ( p = 0.0002) at 7 dpi when normalized to naïve tissue. (G) Quantitative analysis of P2Y2 showing basal levels of expression at 14 dpi when normalized to naïve tissue. (I) Quantitative analysis showing significant downregulation of the 25 kDa isoform within the rostral ( p = 0.0073), injury ( p = 0.0015), and caudal site ( p = 0.0002) at 21 dpi when normalized to naïve tissue. (J) Heat map showing RNA‐seq analysis of P2Y2 gene transcripts compared to highly up‐ and down‐regulated genes in rostral and caudal tissue at 7 and 14 dpi. (K, L) Volcano plot of the zebrafish transcriptome at 7 dpi with P2Y2 transcripts and highly up‐ and down‐regulated genes labeled in rostral and caudal tissue, respectively. Total protein used as loading control. One‐way ANOVA with Dunnett's post hoc test was used for western blot analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.005. ***significant differences: p ≤ 0.001. For RNA‐seq analysis, calculated adjusted p ‐values < 0.05 and a log2(fold change) < −1 or > 1 were considered differentially expressed. **** Significant differences: p ≤ 0.0001.

    Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

    Techniques: Expressing, Western Blot, Tissue, Positive Control, Blocking Assay, Negative Control, Analysis, RNA Sequencing, Labeling, Control

    Behavioral analysis following P2Y2 inhibition at 14 dpi and 21 dpi. (A) Schematic representation of treatment dosages, timeline, and behavioral collection. (B) Fish spent 5 min being recorded unstimulated and uninterrupted in an “open field” paradigm. (C) Fish spent 5 min in the open field chamber with a single “tail touch” stimulation every 30 s. (D) Fish were recorded swimming in a swim tunnel against increasing flow speeds until exhaustion (10 s against the back of the swim tunnel). (E) Swim tunnel paradigm indicating swim tunnel water flow speeds (body length s −1 ; 1 body length = 2 cm) over time (min). The paradigm continues until the fish is exhausted, or 60 min have passed. (F, J) Total distance traveled during the open field paradigm (B) represented as % values of each individual's naïve trial. (G, K) Average degree changes in mean body angle during the tail touch paradigm (C) represented as a % value of each individual's naïve trial. (H, L) Average rostral compensation (tail: Nose movement ratio) during the tail touch paradigm (C) represented as a % value of each individual's naïve trial. (I, M) Time to exhaustion in the swim tunnel paradigm (D, E) represented as a % value of each individual's naïve trial. At 14 dpi (I) the AR‐C group showed a significantly decreased time to exhaustion ( n = 12, p = 0.0427) when compared to the saline group ( n = 10). Unpaired t test (G, H, J–L) and lognormal unpaired t test: Welch variant (F, I, M) used for analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05.

    Journal: The FASEB Journal

    Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

    doi: 10.1096/fj.202504890RR

    Figure Lengend Snippet: Behavioral analysis following P2Y2 inhibition at 14 dpi and 21 dpi. (A) Schematic representation of treatment dosages, timeline, and behavioral collection. (B) Fish spent 5 min being recorded unstimulated and uninterrupted in an “open field” paradigm. (C) Fish spent 5 min in the open field chamber with a single “tail touch” stimulation every 30 s. (D) Fish were recorded swimming in a swim tunnel against increasing flow speeds until exhaustion (10 s against the back of the swim tunnel). (E) Swim tunnel paradigm indicating swim tunnel water flow speeds (body length s −1 ; 1 body length = 2 cm) over time (min). The paradigm continues until the fish is exhausted, or 60 min have passed. (F, J) Total distance traveled during the open field paradigm (B) represented as % values of each individual's naïve trial. (G, K) Average degree changes in mean body angle during the tail touch paradigm (C) represented as a % value of each individual's naïve trial. (H, L) Average rostral compensation (tail: Nose movement ratio) during the tail touch paradigm (C) represented as a % value of each individual's naïve trial. (I, M) Time to exhaustion in the swim tunnel paradigm (D, E) represented as a % value of each individual's naïve trial. At 14 dpi (I) the AR‐C group showed a significantly decreased time to exhaustion ( n = 12, p = 0.0427) when compared to the saline group ( n = 10). Unpaired t test (G, H, J–L) and lognormal unpaired t test: Welch variant (F, I, M) used for analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05.

    Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

    Techniques: Analysis, Inhibition, Saline, Variant Assay, Injection

    Immunohistochemistry showing PCNA and P2Y2 in transverse spinal cord cross sections. (A–C′) PCNA and P2Y2 distributions in naïve spinal cord tissue. (D–F′) PCNA and P2Y2 distributions 7 dpi on the rostral side of the injury. (G–I′) PCNA and P2Y2 distributions 7 dpi on the caudal side of the injury. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Arrows indicate co‐localization; dotted lines outline clearly definable membrane boundaries visible in the P2Y2 channel, with PCNA+ nuclei.

    Journal: The FASEB Journal

    Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

    doi: 10.1096/fj.202504890RR

    Figure Lengend Snippet: Immunohistochemistry showing PCNA and P2Y2 in transverse spinal cord cross sections. (A–C′) PCNA and P2Y2 distributions in naïve spinal cord tissue. (D–F′) PCNA and P2Y2 distributions 7 dpi on the rostral side of the injury. (G–I′) PCNA and P2Y2 distributions 7 dpi on the caudal side of the injury. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Arrows indicate co‐localization; dotted lines outline clearly definable membrane boundaries visible in the P2Y2 channel, with PCNA+ nuclei.

    Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

    Techniques: Immunohistochemistry, Tissue, Membrane, Immunofluorescence

    The impact of P2Y2 manipulation on proliferative cell counts at 7 dpi. (A) Schematic representation of treatment dosages and timeline. (B) For a naïve/non‐injured fish, 450 μm of thoracic spinal cord was analyzed. (C) For injured fish, 450 μm thoracic spinal cord immediately adjacent to the injury was analyzed from rostral and caudal sides. (D, E) Immunohistochemistry showing PCNA in transverse spinal cord cross sections in naïve fish. (F) Representative trace of PCNA + expression along the length of the spinal cord in the experimental conditions ( n = 1 per treatment). (G, H) Representative immunohistochemistry showing PCNA in transverse spinal cord cross sections from the rostral and caudal sides, respectively, of 7 dpi saline treated fish. (I, J) Quantitative analysis of mean PCNA + cell counts around the central canal within the first 450 μm rostral and caudal of the injury, normalized to naïve controls. AR‐C and saline vehicle groups significantly differed from naïve, both rostral (I; saline: p < 0.0001; AR‐C: p < 0.0001) and caudal (J; saline: p = 0.0005; AR‐C: p = 0.0002) of the injury. (K, L) Representative immunohistochemistry showing PCNA in transverse spinal cord cross sections taken from the rostral and caudal sides, respectively, of 7 dpi AR‐C treated fish. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Two‐way ANOVA (F) and one‐way ANOVA (IJ) used for analysis. Data presented as means ± SEM. ***Significant differences: p ≤ 0.001.

    Journal: The FASEB Journal

    Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

    doi: 10.1096/fj.202504890RR

    Figure Lengend Snippet: The impact of P2Y2 manipulation on proliferative cell counts at 7 dpi. (A) Schematic representation of treatment dosages and timeline. (B) For a naïve/non‐injured fish, 450 μm of thoracic spinal cord was analyzed. (C) For injured fish, 450 μm thoracic spinal cord immediately adjacent to the injury was analyzed from rostral and caudal sides. (D, E) Immunohistochemistry showing PCNA in transverse spinal cord cross sections in naïve fish. (F) Representative trace of PCNA + expression along the length of the spinal cord in the experimental conditions ( n = 1 per treatment). (G, H) Representative immunohistochemistry showing PCNA in transverse spinal cord cross sections from the rostral and caudal sides, respectively, of 7 dpi saline treated fish. (I, J) Quantitative analysis of mean PCNA + cell counts around the central canal within the first 450 μm rostral and caudal of the injury, normalized to naïve controls. AR‐C and saline vehicle groups significantly differed from naïve, both rostral (I; saline: p < 0.0001; AR‐C: p < 0.0001) and caudal (J; saline: p = 0.0005; AR‐C: p = 0.0002) of the injury. (K, L) Representative immunohistochemistry showing PCNA in transverse spinal cord cross sections taken from the rostral and caudal sides, respectively, of 7 dpi AR‐C treated fish. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Two‐way ANOVA (F) and one‐way ANOVA (IJ) used for analysis. Data presented as means ± SEM. ***Significant differences: p ≤ 0.001.

    Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

    Techniques: Immunohistochemistry, Expressing, Saline, Analysis, Cell Characterization, Immunofluorescence

    Immunohistochemistry showing HuC/D and P2Y2 in transverse spinal cord cross sections. (A–C′) HuC/D and P2Y2 distributions in naïve spinal cord tissue. (D–F′) HuC/D and P2Y2 distributions 14 dpi on the rostral side of the injury. (G–I′) HuC/D and P2Y2 distributions 14 dpi on the caudal side of the injury. Dorsal surface of the slice are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Arrows indicate examples of co‐localization.

    Journal: The FASEB Journal

    Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

    doi: 10.1096/fj.202504890RR

    Figure Lengend Snippet: Immunohistochemistry showing HuC/D and P2Y2 in transverse spinal cord cross sections. (A–C′) HuC/D and P2Y2 distributions in naïve spinal cord tissue. (D–F′) HuC/D and P2Y2 distributions 14 dpi on the rostral side of the injury. (G–I′) HuC/D and P2Y2 distributions 14 dpi on the caudal side of the injury. Dorsal surface of the slice are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Arrows indicate examples of co‐localization.

    Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

    Techniques: Immunohistochemistry, Tissue, Immunofluorescence

    The impact of P2Y2 manipulation on pan‐neuronal cell counts at 14 dpi. (A) Schematic representation of treatment dosages and timeline. (B) For a naïve/non‐injured fish, 450 μm of thoracic spinal cord was collected. (C) For injured fish, 450 μm thoracic spinal cord was collected immediately adjacent to the injury site on the rostral side, and 450 μm caudal. (D, E) Immunohistochemistry showing HuC/D in transverse spinal cord cross sections in naïve fish. (F) Representative trace of HuC/D + expression along the length of the spinal cord in the experimental conditions ( n = 1 per treatment). (G, H) Representative immunohistochemistry showing taken from the rostral and caudal sides of injury, respectively, of 14 dpi saline treated fish. (I) Quantitative analysis of mean rostral HuC/D + cell counts within the first 450 μm normalized to naïve controls, showing AR‐C significantly reduced HuC/D+ cell counts compared to the saline control group ( p = 0.0459). (J) Quantitative analysis of mean caudal HuC/D + cell counts around the central canal within the first 450 μm normalized to naïve controls, showing AR‐C significantly reduced HuC/D+ cell counts compared to the saline control group ( p = 0.0078). (K, L) Representative immunohistochemistry showing HuC/D in transverse spinal cord cross sections taken from the rostral and caudal sides of injury, respectively, of 14 dpi AR‐C treated fish. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Two‐way ANOVA (F) and one‐way ANOVA (IJ) used for analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.01.

    Journal: The FASEB Journal

    Article Title: Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish

    doi: 10.1096/fj.202504890RR

    Figure Lengend Snippet: The impact of P2Y2 manipulation on pan‐neuronal cell counts at 14 dpi. (A) Schematic representation of treatment dosages and timeline. (B) For a naïve/non‐injured fish, 450 μm of thoracic spinal cord was collected. (C) For injured fish, 450 μm thoracic spinal cord was collected immediately adjacent to the injury site on the rostral side, and 450 μm caudal. (D, E) Immunohistochemistry showing HuC/D in transverse spinal cord cross sections in naïve fish. (F) Representative trace of HuC/D + expression along the length of the spinal cord in the experimental conditions ( n = 1 per treatment). (G, H) Representative immunohistochemistry showing taken from the rostral and caudal sides of injury, respectively, of 14 dpi saline treated fish. (I) Quantitative analysis of mean rostral HuC/D + cell counts within the first 450 μm normalized to naïve controls, showing AR‐C significantly reduced HuC/D+ cell counts compared to the saline control group ( p = 0.0459). (J) Quantitative analysis of mean caudal HuC/D + cell counts around the central canal within the first 450 μm normalized to naïve controls, showing AR‐C significantly reduced HuC/D+ cell counts compared to the saline control group ( p = 0.0078). (K, L) Representative immunohistochemistry showing HuC/D in transverse spinal cord cross sections taken from the rostral and caudal sides of injury, respectively, of 14 dpi AR‐C treated fish. Dorsal surface of the slices are oriented to the top of the image, the asterisk marks the central canal, and the white scale bar represents 50 μm. Two‐way ANOVA (F) and one‐way ANOVA (IJ) used for analysis. Data presented as means ± SEM. *Significant differences: p ≤ 0.05. **Significant differences: p ≤ 0.01.

    Article Snippet: All tissue sections were then incubated at room temperature overnight in a 0.1% Triton X‐100/0.01 M PBS solution containing at least one of the following primary antibodies: P2Y2 (Rabbit polyclonal, 1:200, Almone labs: APR010AN0802, RRID: AB_2040078), GFAP (Chicken polyclonal, 1:2000, ThermoFisher Scientific: PA1‐10004, RRID: AB_1074620), PCNA (Mouse monoclonal, 1:1000, Abcam: ab29, RRID: AB_303394), or HuC/D (Mouse Monoclonal, 1:20 000, ThermoFisher Scientific: A‐21271, RRID: AB_221448).

    Techniques: Immunohistochemistry, Expressing, Saline, Analysis, Control, Cell Characterization, Immunofluorescence